Fig. 1 | Scientific Reports

Fig. 1

From: Mitochondrial transplantation normalizes transcriptomic and proteomic shift associated with ischemia reperfusion injury in neonatal hearts donated after circulatory death

Fig. 1

Graphic representation of the study outline. Neonatal (3–4.5 kg) or pediatric (10–15 kg) female Yorkshire pigs underwent cardiac death by induction of deep anesthesia and neuromuscular blockade and extubation. Hearts were harvested and received either vehicle or mitochondria and underwent ex situ heart perfusion. A group of sham hearts underwent ex situ heart perfusion but not the part of the cardiac death. Tissue was collected and snap frozen in the end of the perfusion and RNA seq and SOMAscan were performed. Left ventricle developed pressure (mmHg) and infarct size (% of total cardiac mass) following 2 h. of unloaded and 2 h. of loaded ex-situ heart blood perfusion for sham control hearts receiving no warm ischemia (SHAM, blue), DCD hearts receiving vehicle alone (VEH, black) and DCD hearts receiving vehicle containing 5 × 109 mitochondria (MITO, red). VEH and MITO were both delivered as a bolus antegrade into the aortic root over 5 s. (Figures retrieved from Alemany, et al.). *p < 0.05 for Mitochondria vs Vehicle groups; VEH: Vehicle group; MITO: Mitochondria group; SHAM: Sham group.

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