Fig. 2

(A) Heatmap of transcriptomic regulation patterns in neonatal DCD Mitochondria (Mitochondria), DCD Vehicle (Vehicle) and Sham (DBD) control hearts. Τhe log fold change of gene expression is shown with pseudocolor scale (-2 to 2), with blue denoting downregulation and orange denoting upregulation when comparing hearts that received mitochondria to vehicle. Columns represent FC comparisons, and rows represent the genes. Neonatal experimental groups are indicated. (B) Principal component analysis (PCA) for neonatal hearts receiving mitochondria (red circles) or vehicle alone (green circles). Sham indicated in blue. (C) Volcano plot indicating the downregulated (blue) and upregulated (red) genes in the neonatal mitochondria group compared to vehicle. (D) Gene ontology (GO) pathway analysis indicating downregulated and upregulated biological process in the neonatal mitochondria group compared to vehicle. The size of the dot correlates with the number of genes and the color with the fold change. (E) Enrichment plot analysis indicating downregulated and upregulated pathways in the neonatal mitochondria group compared to vehicle. NES: Normalized enrichment plot; FDR: False discovery rate. (F) Gene ontology analysis indicating the most significant cellular compartment and molecular function in the neonatal mitochondria group compared to vehicle. (G) Gene ontology molecular function analysis indicating the top 200 genes in the neonatal mitochondria group compared to vehicle. (H) Network analysis indicating the top hub genes in significantly downregulated and upregulated pathways in the neonatal mitochondria group compared to vehicle. (I) Gene networks in upregulated and downregulated pathways in the neonatal mitochondria group compared to vehicle. All results are shown following 4 h perfusion, consisting of 2 h. unloaded and then 2 h of isovolumic loaded heart perfusion for each group.