Fig. 3

(A) Heatmap of proteomic regulation patterns in neonatal DCD Mitochondria (Mitochondria), DCD Vehicle (Vehicle) and Sham (DBD) control hearts. Τhe log fold change of gene expression is shown with pseudocolor scale (-2 to 2), with blue denoting downregulation and orange denoting upregulation. Columns represent FC comparisons, and rows represent the proteins. Neonatal experimental groups are found on the bottom. (B) Principal component analysis (PCA) for neonatal hearts receiving mitochondria (red circles) or vehicle alone (green circles). Sham indicated in blue. (C) Volcano plot indicating the downregulated (blue) and upregulated (red) proteins in the neonatal mitochondria group compared to vehicle. (D) Manhattan plot of two databases (Gene Ontology (GO) Cellular Component 2018 and Jensen Compartments) indicating the most significant GO cellular compartments when comparing neonatal mitochondria group to vehicle. (E) Barplots indicating upregulated and downregulated GO biological processes in the neonatal mitochondria group compared to vehicle. (F) Barplot indicating upregulated and downregulated GO molecular function in the neonatal mitochondria group compared to vehicle. (G) Dotplot indicating disease associated enrichment analysis when comparing neonatal mitochondria group to vehicle. (H) Dotplot indicating tissue/cell enrichment analysis when comparing neonatal mitochondria group to vehicle. (I) Venn diagram depicting the number and name of overlapping genes as well as the respective processes of the RNA seq and proteomics data when comparing neonatal mitochondria group to vehicle. (J) Common predicted upstream transcription factors (TF) in RNA seq and proteomics data when comparing neonatal mitochondria group to vehicle. Interaction network of ribosome biogenesis (orange) and immune response (blue) also shown. FDR: False discovery rate. All results are shown following 4 h perfusion, consisting of 2 h. unloaded and then 2 h of isovolumic loaded heart perfusion for each group.