Fig. 4

(A) Heatmap of transcriptional regulation patterns at the end of the perfusion period. Τhe log fold change of gene expression is shown with pseudocolor scale (-2 to 2), with blue denoting downregulation and orange denoting upregulation. Columns represent FC comparisons, and rows represent the genes. Pediatric experimental groups are found on the bottom. (B) Principal component analysis (PCA) for pediatric hearts receiving mitochondria (red circles) or vehicle alone (green circles). Sham indicated in blue. (C) Volcano plot indicating the downregulated (blue) and upregulated (red) genes in the pediatric mitochondria group compared to vehicle. (D) Gene ontology (GO) pathway analysis indicating downregulated and upregulated biological process in the pediatric mitochondria group compared to vehicle. The size of the dot correlates with the number of genes and the color with the fold change. (E) Barplot indicating common domains of differentially expressed genes (DEGs) when comparing pediatric mitochondria group with vehicle. (F) Barplots indicating significant Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways when comparing pediatric mitochondria group with vehicle. (G) Enrichment plot analysis indicating downregulated and upregulated pathways in the pediatric mitochondria group compared to vehicle. NES: Normalized enrichment plot; FDR: False discovery rate.