Fig. 5

(A) Heatmap of proteomic regulation patterns at the end of the perfusion period. Τhe log fold change of gene expression is shown with pseudocolor, with blue denoting downregulation and orange denoting upregulation. Columns represent FC comparisons, and rows represent the proteins. Pediatric experimental groups are found on the bottom. (B) Principal component analysis (PCA) for pediatric hearts receiving mitochondria (red circles) or vehicle alone (green circles). Sham indicated in blue. (C) Heatmap of proteomic inflammatory alterations at the end of the perfusion period. Τhe log fold change of gene expression is shown with pseudocolor, with blue denoting downregulation and orange denoting upregulation. Columns represent FC comparisons, and rows represent the proteins. Pediatric experimental groups are found on the bottom. (D) Volcano plot indicating the downregulated (blue) and upregulated (red) proteins in the pediatric mitochondria group compared to vehicle. (E) Manhattan plot of two databases (Gene Ontology (GO) Cellular Component 2018 and Jensen Compartments) indicating the most significant GO cellular compartments when comparing pediatric mitochondria group to vehicle. (F) Dotplot indicating significant gene ontology (GO) biological processes when comparing pediatric mitochondria group to vehicle. (G) Protein networks in enrichment pathways when comparing neonatal mitochondria group to vehicle. FDR: False discovery rate. (H) Venn diagram depicting the number and name of overlapping genes as well as the respective processes of the RNA seq and proteomics data when comparing pediatric mitochondria group to vehicle. (I) Protein–protein interaction network for cell cycle, mitochondrion organization, heart development, muscle organ development and gluconeogenesis when comparing neonatal mitochondria group to vehicle. FDR: False discovery rate.