Fig. 1

Schematic overview of the experimental design, showing the selected timepoints for sample collection (− 7, 3, 6, 9, 21 relative to calving). EDTA blood samples were tested for telomere length. Heparin blood samples were analysed for oxidative markers (GSSG, GPx, MDA, ORAC, SOD) and inflammation (Hp and SAA). Serum and sodium fluoride blood samples were used for the analysis of the metabolic markers (glucose, BHB, NEFA, IGF-1, insulin, fructosamine, AG ratio, Albumin, α-globulin, β-globulin, γ-globulin, Total protein, Urea, AST). Milk samples for measuring BHB and Urea were collected daily for 21 days, excluding the first two days following parturition. (Number of animals = 61).