Fig. 1
From: An epigenetic memory at the CYP1A gene in cancer-resistant, pollution-adapted killifish
The epigenetic memory at CYP1A in PAH-tolerant Fundulus heteroclitus. (A) Experimental design schematic for depuration of wild-caught fish, manual fertilization of embryos, and exposure to PAH. (B) Xenobiotic response elements (XREs) in the CYP1A proximal promoter-enhancer are conserved across four fish (one male and one female each from PAH-tolerant and PAH-sensitive populations). (C–E) Relative fold-change values for embryonic gene expression for three AHR target genes (CYP1A, CYP1B, and CYP1C). Figures show C(t) values from PAH-challenged embryo samples normalized to the average control C(t). **P-value < 0.01 from independent samples t-tests comparing C(t) values from PAH-challenged embryos to control embryos within each group. Hedge’s g values represent effect sizes for difference-in-difference (D-i-D) tests [PAH-tolerant (treatment-control)] – [PAH-sensitive (treatment-control)]. Hedge’s g = 0.2 is a small effect, Hedge’s g = 0.4–0.6 is a moderate effect, Hedge’s g = or > 0.8 is a large effect. 95% confidence intervals that do not cross zero reflect p < 0.05. Dpf – days post-fertilization, PAH – polycyclic aromatic hydrocarbons, D-i-D – Difference-in-difference, XRE – xenobiotic response element, TSS – transcription start site.
