Fig. 2 | Scientific Reports

Fig. 2

From: Investigating the effects of stereotactic body radiation therapy on pancreatic tumor hypoxia and microvasculature in an orthotopic mouse model using intravital fluorescence microscopy

Fig. 2

Experiment design. (a) Schematic timeline of BxPC3 cell inoculation, surgical implantation of pancreatic imaging window (PIW), SBRT treatment, and schedule for serial intravital imaging. Blue arrows indicate the number of days before (−) and after (+) SBRT treatment. Intravital imaging was performed 1 day before and 1, 4, 7 and 14 days post-SBRT. Mice were euthanized at days +1 and +14 for tissue collection and immunohistochemical (IHC) staining. (b) SBRT given in 5 daily fractions of 8 Gy (5 × 8 Gy) using an X-Rad 225Cx micro-irradiator. Inset image shows the mouse positioned on its right flank on a custom, 3D-printed stage (also pictured in Fig. 1E). The custom stage minimizes motion artifacts and ensures that the irradiated and imaging regions are identical.

Back to article page