Fig. 3 | Scientific Reports

Fig. 3

From: Investigating the effects of stereotactic body radiation therapy on pancreatic tumor hypoxia and microvasculature in an orthotopic mouse model using intravital fluorescence microscopy

Fig. 3

Intravital fluorescence microscopy (IVFM) enables longitudinal monitoring of orthotopic BxPC3 tumors in vivo. Top row (a) shows representative fluorescence images of non-irradiated BxPC3 tumors in one mouse. Bottom row (b) shows representative fluorescence images of BxPC3 tumors treated with 5 × 8 Gy SBRT in another mouse. BxPC3 cells (DsRed) are shown in red and tumor cell HIF activity (5xHRE/GFP) in green. White arrows indicate regions of BxPC3 tumor cell HIF activity. In vivo labeling of tumor microvasculature was performed by intravenous injection of APC-conjugated CD31 antibody (cyan). Scale bar = 500 μm.

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