Fig. 4. | Scientific Reports

Fig. 4.

From: Investigating the effects of stereotactic body radiation therapy on pancreatic tumor hypoxia and microvasculature in an orthotopic mouse model using intravital fluorescence microscopy

Fig. 4.

5 × 8 Gy SBRT decreases pancreatic tumor cell proliferation. (a) Intravital fluorescence microscopy reveals a persistent decrease in the DsRed mean fluorescence intensity (MFI) of DsRed-BxPC3 tumor cells up to 14 days post-SBRT (grey bars) compared to non-irradiated BxPC3 tumor cells (white bars). (b) Representative images and (c) quantification of γH2AX staining (brown) in ex vivo BxPC3 tumor tissue sections show significant DNA damage in tumor cells 1 day post-SBRT, compared to non-irradiated control mice. No significant differences in γH2AX staining are seen 14 days after treatment. (d) Representative images and (e) quantification of Ki67 expression (brown) shows a significant decrease in tumor cell proliferation 14 days post-SBRT, compared to non-irradiated animals. Tissue sections are counterstained with hematoxylin (blue). Scale bar = 200 μm. n = 7 mice per group. *p < 0.05, **p < 0.01, ***p < 0.001.

Back to article page