Table 1 Oxygenation, oxidation reactions and hemolysis in RBCs treated with curcumin. Hemolysis was monitored spectrophotometrically by quantify free hemoglobin in solutions10. Spontaneous oxidation (autoxidation) of hemoglobin RBCs (60µM) in AS-3 solutions at pH (7.4) and room temperature followed for 35 days. Spectral analysis in the visible region of RBC solutions were followed spectrophotometrically in Agilent Spectrophotometer. Oxygen dissociation curves (ODCs) and the Hill coefficient of RBCs were obtained using the Hemox AnalyzerTM (TCS Scientific, New Hope, PA). Samples from the stored blood were taken, washed, packed, and were re-suspended in plasma to a hematocrit of approximately 20%. Approximately 120 μL of each suspension was added to 3 ml of Hemox buffer, pH 7.4, in a cuvette and subjected to ODC analysis at 37 °C.
