Fig. 4

Metabolic pathway mapping significantly differs in rumen fluid (A) and serum (B) (n = 10) metabolites under optimum temperature period and high temperature period conditions. The pathway analysis was performed using Metaboanalyst 5.0 program. The x-axis and y-axis represent pathway impact value and -log 10 P value, respectively. The results are presented graphically as a bubble plot. The darker color and larger size represent higher P value from enrichment analysis and greater impact from pathway topology analysis, respectively. Metabolic pathway names: A = phenylalanine, tyrosine and tryptophan biosynthesis; B = phenylalanine metabolism; C = alanine, aspartate and glutamate metabolism; D = glycine, serine and threonine metabolism; E = cysteine and methionine metabolism; F = glyoxylate and dicarboxylate metabolism; G = citrate cycle (TCA cycle); H = arginine and proline metabolism; I = glutathione metabolism; J = pentose and glucuronate interconversions; K = ascorbate and aldarate metabolism; L = biotin metabolism; M = starch and sucrose metabolism; N = histidine metabolism; O = tryptophan metabolism; P = tyrosine metabolism.