Fig. 5
From: A new perspective on bone development in vitamin D deficiency-associated obese children
Validation of glycerol ester pathway-related proteins and gene expression. (A) Venn diagram summarising changes in lipid metabolites on a vitamin D-deficient diet. (B) The common differential metabolite in children and mice was triglycerides. (C) Masson staining of femur (scale bar, 20 μm) for quantitative evaluation of new bone (n = 6, mean ± SEM; *p < 0.05,** < 0.01,***p < 0.001). (D) Western-blotting showing RUNX2, COL-1 protein levels in ND and VDD groups. (n = 3, mean ± SEM; *p < 0.05,** < 0.01,***p < 0.001). (E) Western-blotting showing GPAT4, AGPAT1, DGAT2 protein levels in ND and VDD groups. (n = 3, mean ± SEM; *p < 0.05,** < 0.01,***p < 0.001) (F) Real-time quantitative PCR showing the levels of RUNX2, COL-1 genes in ND and VDD groups. (n = 6, mean ± SEM; *p < 0.05,** < 0.01,***p < 0.001) (G) Real-time quantitative PCR showing the levels of GPAT4, AGPAT1, DGAT2 genes in ND and VDD groups. (n = 6, mean ± SEM; *p < 0.05,** < 0.01,***p < 0.001) (H) Triglyceride synthesis pathway.
