Fig. 2

In silico model fitting to experimental data. (a) Experimental protocols of published in vivo mouse model studies we used for model fitting. Shaded regions indicate HDM challenge present and stars represent the timings of experimental data measurements (weeks from birth). (b) Model simulations of the parameterised in silico model for each of the experimental protocols. The data obtained for each protocol are (1–2) epithelial barrier damage (IL-33)¹⁵, (3) airway resistance¹⁶, and (4) eosinophil cell counts¹⁶ from a neonatal mouse model, and (5) eosinophil cell counts and airway resistance¹⁷ from an adult mouse model. Shaded regions indicate HDM challenge present and the dots with error bars indicate the mean data measurements with SEM. The green lines indicate the fit to PBS data and the dark pink lines the fit to HDM data. The dotted horizontal lines in the epithelial damage and eosinophilic inflammation plots indicate the thresholds for the remodelling and sensitisation switches, respectively. The resulting switch states are shown by the colours of the bars above the plots, with the on-states represented by pink and orange for remodelling and sensitisation switches and the off-states represented by white.