Fig. 12 | Scientific Reports

Fig. 12

From: Pt-LIS1 participates nuclear deformation and acrosome formation via regulating Dynein-1 during spermatogenesis in Portunus trituberculatus

Fig. 12

Colocalization of Pt-LIS1 and α-Tubulin proteins during spermatogenesis was diminished after Pt-Lis1 silencing in vivo. (A1-A5) Spermatogonia; (B1-B5) Secondary spermatocytes; (C1-C5) Early spermatids; (D1-D5) Middle spermatids; (E1-E5) Late spermatids; (F1-F5) Mature sperm. The blue color indicates the nuclei stained with DAPI; the red color indicates Pt-LIS1; the green color indicates α-Tubulin, and the arrows indicated that the typical signals of Pt-LIS1 and Pt-DIC. N: nuclear; AC: acrosome cap; AT: acrosome tube; MC: membrane complex; FL: fibrous layer; ML: intermediate layer; LS: lamellar structure; RA: radial arm. Scale bar = 10 μm.

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