Fig. 5

Colocalization distribution of Pt-LIS1 and Pt-DIC proteins during spermatogenesis. (A1-A5) Spermatogonia; (B1-B5) Secondary spermatocytes; (C1-C5) Early spermatids; (D1-D5) Middle spermatids; (E1-E5) Late spermatids; (F1-F5) Mature sperm. The blue color indicates the nuclei stained with DAPI; the green color indicates Pt-LIS1; the red color indicates Pt-DIC; and the arrows indicated that the typical signals of Pt-LIS1 and Pt-DIC. N: nuclear; AC: acrosome cap; AT: acrosome tube; MC: membrane complex; FL: fibrous layer; ML: intermediate layer; LS: lamellar structure; RA: radial arm. Scale bar = 10 μm.