Fig. 9 | Scientific Reports

Fig. 9

From: Pt-LIS1 participates nuclear deformation and acrosome formation via regulating Dynein-1 during spermatogenesis in Portunus trituberculatus

Fig. 9

Knockdown efficiency of Pt-Lis1 by dsRNA injection in vivo. (A) Synthesis of specific dsRNA, egfp-dsRNA (463 bp), Pt-Lis1-dsRNA1 (435 bp) and Pt-Lis1-dsRNA2 (441 bp), the original blot is presented in Supplementary Fig. 4. (B) The results of qPCR showed that the relative expression of Pt-Lis1 mRNA was significantly decreased after dsRNA interference. (C) WB detection of Pt-LIS1 expression in the testis, original blots are presented in Supplementary Fig. 5, 6. (D) ImageJ software was used to extract the grayscale values of the protein bands, and Pt-LIS1 protein expression was significantly reduced after interference with Pt-Lis1. (E–H) The relative expression of Pt-Lis1 mRNA in the testis and vas deferens was significantly reduced. “ns” means no significant difference, P > 0.05, “*” means a significant difference, P < 0.05, “**” means an extremely significant difference, P < 0.01.

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