Fig. 2
Design and mass distribution analysis of mTfRPro119 used for cell-free in vitro assays. (a) Full size murine TfR (mTfR)7 consists of an extracellular (EC), transmembrane (TM), and intracellular (IC) domain where the TM domain cannot easily be produced recombinantly in solution. mTfRPro119 comprising of the mTfR ectodomain is commonly used as TfR construct for in vitro assays. (b) SDS-PAGE with Coomassie staining of mTfR Pro119 under non-reducing and reducing (r) conditions (1 µg protein/lane). The complete gel can be seen in Supplementary Fig. S2d. (c) Mass distribution of purified mTfR Pro119 at 119 nM in PBS measured by mass photometry. Peak volumes represent counts of molecules of a defined mass (kDa). The first peak likely represents buffer impurities.
