Fig. 5
In vitro BBB assay comparing transcytosis efficiency of RmAb158-scFv8D3 variants, 8D3 and scFv8D3-scFc across a BBB endothelial cell monolayer. (a) Schematic illustration of experimental setup with cEND cells grown on porous cell culture inserts treated with 13.3 nM or 266 nM antibodies for 1 h, washed and incubated in fresh medium for 6 h. Average antibody concentrations in “chase” samples taken from the basolateral compartment after 6 h were measured by a sandwich ELISA (b,c). Results are presented as means with 95% confidence intervals. One-way ANOVA with Tukey multiple comparison was applied. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns: p > 0.05). (6 wells per condition). The raw data can be found in Supplementary Table S5.
