Table 2 Parameters of the tomographic acquisition, phase retrieval, ring removal (post processing), and stitching, listed for the two ID19 and the ID17 configurations. The X-rays are generated by an undulator (U) at ID19 and by a wiggler at ID17 (W). The phase retrieval was performed via the contrast-transfer-function (CTF) approach or the generalized Paganin method (GPM)56.
From: Three-dimensional structure of entire hydrated murine hearts at histological resolution
ID19 conf.1 | ID19 conf.2 | ID17 | |
|---|---|---|---|
Acquistion parameter | |||
Photon energy E [keV] | 19 (U) | 19 (U) | 50 (W) |
Monochromaticity\(\frac{\Delta E}{E}\) | 10\(^{-2}\) | 10\(^{-2}\) | Broad spectrum |
Detector FOV [\(mm^{2}\)] | 4.1 x 3.2 | 1.7 x 1.4 | 9.2x 2.3 (beam height) |
Pixelsize [\(\mu m\)] | 1.6078 | 0.6493 | 3.58 |
Source sample distance\(z_1\)[m] | 145 | 145 | 150 |
Propagation distance\(z_2\)[mm] | 270 | 170 | 1000 |
Fresnel number F | 0.15 | old / new 0.038 | 0.5169 |
Exposure time [ms] | 100 | 50 | 35 |
Projections | 1500 | 1500 | 4001 |
Angular range [\(^{\circ }\)] | 0–180 | 0–180 | 0–360 |
Phase retrieval | |||
Method | CTF | CTF old | GPM |
\(\alpha _{1}\) | \(0.04404*10^{-4}\) | \(0.00069*10^{-4}\) | - |
\(\alpha _{2}\) | 0.288 | 0.288 | - |
\(\frac{\delta }{\beta }\) | 1400 | 1400 | 130 |
Ring removal | |||
Method | Wavelet | Wavelet | Wavelet |
Wavelet name | sym16 | sym16 | sym8 |
DecNum | 7 | 7 | 7 |
Sigma | 4 | 4 | 3 |
Stitching | |||
Number of tomograms | 20 | 120 | 4–6 |
Highpass filter kernel [px] | 8 | 16 | 300 |
