Fig. 2

Deficiency of PRDX6 promotes apoptosis and inhibits proliferation in vitro. (A,B) The efficiency of transfection and knock down was assessed by western blotting and qRT-PCR. (C) Cell viability was detected after deficiency using the CCK-8 assay. (D) Cell apoptosis was detected after deficiency using the Annexin V/7-AAD double staining. (E) Cell proliferation was detected after deficiency using the EdU staining. (F) Cell cycle was detected after deficiency using the PI staining. The data are the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.