Fig. 2

Circ_0043256 regulated CoCl2-induced proliferation, migration, and apoptosis of MKN-45 and AGS cells. MKN-45 and AGS cells were exposed to different concentrations (50 µM, 100 µM, 200 µM, and 300 µM) of CoCl2 to induce a hypoxic environment. (A–C) Western blot analysis was performed to detect the expression levels of glycolytic related proteins HK2 and GLUT1 in MKN-45 and AGS cells induced by different concentrations of CoCl2. β-actin is a loading control. (D) The expression of si-circ_0043256 was quantified using RT-qPCR analysis. ^*P < 0.05 (vs. 0 µM), ^**P < 0.01 (vs. 0 µM). (E) MKN-45 and AGS cells were transfected with si-circ_0043256 and treated with CoCl2. The expression level of si-circ_0043256 was determined through RT-qPCR analysis (F and G) The proliferation of MKN-45 and AGS cells were evaluated by CCK-8 assay. (H–J) MKN-45 and AGS cell migration was tested by Wound-healing assay. Experiments were terminated after scratching for 48 h. (K-M) Flow cytometry was used to detect apoptosis and measure the rate of apoptosis. (N-P) The cell cycle of MKN-45 and AGS cells was examined by flow cytometry. ^*P < 0.05 (vs. siRNA-NC), ^**P < 0.01 (vs. siRNA-NC), ^##P < 0.01 (vs. CoCl2 + siRNA-NC).