Fig. 6

Circ_0043256 regulated CoCl2-induced aerobic glycolysis of MKN-45 and AGS cells by targeting the miR-593-5p/RRM2 axis. MKN-45 and AGS cells were transfected with si-circ_0043256 and/or miR-593-5p mimic to investigate their effects on CoCl2-induced aerobic glycolysis. (A and B) The ATP production, glucose consumption, and lactic acid content in CoCl2-induced MKN-45 and AGS cells were assessed using commercially available kits. (C and D) The protein levels of HIF-1α, HK2, ENO1, LDHA, GLUT1, and PKM2 in AGS cells were determined by Western Blot analysis with β-actin serving as a loading control. (E and F) The protein levels of HIF-1α, HK2, ENO1, LDHA, GLUT1, and PKM2 in MKN-45 cells were measured via Western Blot analysis. β-actin is a loading control. ^**P < 0.01 (vs. siRNA-NC), ^#P < 0.05 (vs. CoCl2 + siRNA-NC), ^##P < 0.01 (vs. CoCl2 + siRNA-NC), ^&P < 0.05 (vs. CoCl2 + si-circ_0043256 + mimic-NC), ^&&P < 0.01 (vs. CoCl2 + si-circ_0043256 + mimic-NC).