Fig. 4
From: Atrophic meibomian gland dysfunction induced by eyelid margin cryotherapy with liquid nitrogen
Histological changes and lipid synthesis of MGs after liquid nitrogen injury of the eyelid margin. (A) Oil red O staining showing the meibomian gland ducts are partially filling. at days 7, 14, 21 and 28 post liquid nitrogen injury (circle). Some oil red O-labeled droplets indicated atrophy acini (black star). Scale bars represent 200 μm (n = 3). (B, C) The expression of PPARγ in immunofluorescence significantly decreases on the 7th, 21st, and 28th days following liquid nitrogen cryopreservation. ****p < 0.0001. (D, E) Western blot analysis shows the decline in PPARγ after liquid nitrogen treatment. The expression reduction was most significant at 7 days and 21days. **p < 0.01, ns > 0.05.
