Fig. 1

Bioinformatics analysis. (A) Box plots of sample calibration at 4 h and 24 h; (B) PCA plots showing sample differences at 4 h and 24 h; (C) Volcano plots of differential genes at 4 h and 24 h; (D) Heatmaps of differential genes at 4 h and 24 h; (E) Bar charts of GO enrichment analysis at 4 h and 24 h; (F) Bubble plots of KEGG enrichment analysis at 4 h and 24 h.