Fig. 6
Irisin increases the expression of phosphorylated AMPK and phosphorylated MerTK proteins, promotes autophagy, and inhibits pyroptosis/inflammatory response. (A) Western blot detection of p-AMPK, p-MerTK, PGC-1α, KLF4, LC3I, and Beclin-1 protein expression in BV2 cells; (B) Western blot detection of NOX4, p-SYK, and Dectin-1 protein expression in BV2 cells from the above seven groups; (C) Q-PCR detection of YM-1, FIZZ, and arginase-1 levels in the seven groups; (D) Q-PCR detection of NLRP3, IL-1β, and TNFα levels in the seven groups; (E) Q-PCR detection of Caspase-1, Caspase-4, and GSDMD levels in the seven groups. GAPDH as control protein. Data are expressed as the mean ± standard deviation. **P<0.01; *P<0.05; nsP>0.05.
