Fig. 5
(A) Protein sequence coverage of the detected proteins in mfge8-/-/gRNA3 (117), BuMEC. (B) Scatter plot of changes in protein abundance in mfge8-/-/gRNA2 (115), mfge8-/-/gRNA3 (117) and mfge8-/-/gRNA4 (116) and control BuMEC. (C) Heat Map presentation of hierarchically clustered differentially expressed proteins in control and mfge8-/-/gRNA3 BuMEC. Red colour depicts high while blue colour depicts low expression levels. (D) Relative expression of MFGE8 protein in mfge8 knock-out BuMEC generated using three different gRNAs and control BuMEC. (E) Venn diagram representing the distribution of proteins in control and mfge8 knock-out cells. Peptides were labelled with iTRAQ labels 114- control, 115- mfge8-/-/gRNA2, 116- mfge8-/-/gRNA4 and 117- mfge8-/-/gRNA3. Control BuMEC, mfge8-/-/gRNA2, mfge8-/-/gRNA3 and mfge8-/-/gRNA4 yielded a total of 4276, 4278, 4282 and 4275 proteins respectively, among these, 4267, 4272 and 4270 proteins were shared by knock-out cells with control cells respectively. F) In- depth proteome analysis of mfge8-/-/gRNA3 and control BuMEC, helped in prediction of localization of identified proteins in cellular compartments of BuMEC.
