Table 2 List of primers used in real time PCR.
Gene | Primer sequence | Annealing temperature (ºC) | Product size (bp) | Accession No. |
|---|---|---|---|---|
Serpin family H member 1 (SERPINH1) | SERPINH1F- GCGGAGAGTGGAATCCGTT SERPINH1R- CCTTGAAACTGCCTCAGGGG | 59 | 120 | XM_005216248.4 |
Fatty acid binding protein 5 (FABP5) | FABP5F- AGTTTCTGTAGGCACTGGGC FABP5R- GGGCTCAACGAGTTGTAGGT | 59 | 172 | NM_174315.3 |
Metallothionein 2 A (MT2A) | MT2AF- AGTTCAGCTCGCCATGGAT MT2AR- AGCAGCTCTTCTTGCAGGAG | 59 | 113 | BC112477.1 |
Apolipoprotein A2 (APOA2) | APOA2F- CCAAGGGCTCAGAGCTTCAA APOA2R- TTAAGCAGGTCAGTCCCAGC | 59 | 100 | NM_001045916.2 |
MFGE8 | MF1- TTCTGTGACTCCAGCCAGTG MR1- CAGTTACCTGGCACTCAGCA | 60 | 175 | MK688380 |
Cas9 | Cas9 F2- CAGCTGGTGCAGACCTACAA Cas9 R2-CGAACAGGCCATTCTTCTTC | 60 | 157 | pSpCas9(BB)-2 A-GFP (PX458) |
