Fig. 1
Evaluation of different PTX concentrations injected in zebrafish embryos. (a) mortality reported in the graph showed a negligible percentage of dead embryos injected at 48 hpf, even with higher PTX concentration (22 µM). (b) sublethal defects average reported is less than 0.5%. The main defect observed in injected embryos is cardiac edema. (c) Representative images of embryos at 48 h post injection showed the frame used to analyze the GFP signal. (Scale bar, 100 µm). (d) graph reporting the fluorescent area measured in arbitrary units. (e) peripheral sensory neurons length measured and reported in graph. The effects of the different PTX concentrations were observed in n = 39 embryos for each experimental group during three different experiments, for a total of n = 195 embryos.
