Fig. 2
Liposomes injections results. (a) Graph shows mortality percentages of embryos injected with liposomes loaded with PTX 30 and 100 µM, compared to embryos injected with PTX alone (30 or 100 µM) and liposomes unloaded observed during 3 independent experiments, for a total of n = 32 embryos for each experimental group. The liposomes unloaded are defined as A, wich are blank liposomes at liposome concentration equivalent to LipoPTX 30 µM, or B, wich are blank liposomes with liposome concentration equivalent to LipoPTX 100 µM. A high mortality percentage (40%) is observed in embryos injected with PTX 30 μM, clearly reduced in embryos injected with LipoPTX 30 μM (12%) (***p < 0.0001). The same is observed in embryos injected with PTX 100 μM (31.6% of mortality), compared to embryos injected with LipoPTX 100 μM (14.6%) (##p < 0.005). (b) Graph reporting the sublethal defects observed. The most recurrent defect is cardiac edema, present in an average of 0.5% of embryos injected. (c, d) Graphs reporting analyses on GFP fluorescent signal and on peripheral sensory neurons length measures at 48 h post injection.
