Fig. 4
Paclitaxel and liposomes injections results in embryos at 48 hpi (a) Graph reporting the mortality average percentage of embryo injected with PTX 30 µM, compared to Ctrl and LipoPTX 30 µM, Lipo unloaded, MSLP-LipoPTX 30 µM and MSLP-Lipo unloaded injected embryos. The number of embryos observed was n = 13 embryos for each group (n total = 80), in 6 different experiments. At 48 hpi, the mortality rate showed a significant increase in embryos injected with PTX 30 µM compared to Ctrl (@p = 0.0002, two-sided), to LipoPTX 30 µM (***p = 0.0008, two-sided), to Lipo unloaded 30 µM (***p = 0.0008, two-sided), to MSLP-LipoPTX 30 µM (#p = 0.0192, two-sided), and to MSLP-Lipo unloaded 30 µM (***p = 0.0008, two-sided). For statistical analyses, the Fisher’s exact test was applied. (b) Graph reporting the negligible percentage of sublethal defects observed (cardiac edemas), also detected in Ctrl not injected. The results showed that either functionalized or not liposomes were well tolerated by the embryos, and their stability in the biological milieu allowed protection against the PTX action in the healthy tissues for the 48-h period observed.
