Fig. 6
Molecular investigations of liposomes effects compared to PTX. (Upper panel) Graphs reporting gene expression levels of nfkb2, tnfα, il1β, il6, tgfβ1a, tgfβ1b and tgfβ3, a panel of genes reported in literature as related to CIPN. The results showed that the genes related to neuropathic pain are generally upregulated in embryos injected with PTX 30 µM, compared to Ctrl embryos and both liposomes injected embryos. nfkb2, a key regulator of molecul es and pathways important for inflammation and pain, is significantly downregulated (@p = 0.022) by LipoPTX 30 µM injection. (Lower panel) Graphs show expression levels of genes related to oxidative stress. The presence of increased ROS is known to be caused by the PTX exposure. Our results showed a decreasing trend in all the gene expression levels observed after injection with liposomes.
