Table 1 Primer sequences used in real-time quantitative polymerase chain reaction.

From: Toxoplasma GRA16 attenuates Tau hyperphosphorylation and enhances autophagy in thrombin-treated HT-22 hippocampal neuronal cells

Gene

Forward primer (5′-3′)

Reverse primer (5′-3′)

CDK5

GTACTCCACGTCCATCGACATG

GCCATTGTTCCTCAGTCGGTGT

ATG12

GAAGGCTGTAGGAGACACTCCT

GGAAGGGGCAAAGGACTGATTC

BNIP3

GCTCCAAGAGTTCTCACTGTGAC

GTTTTTCTCGCCAAAGCTGTGGC

LC3

CTGCCTGTCCTGGATAAGACCA

CTGGTTGACCAGCAGGAAGAAG

BECN1

CAGCCTCTGAAACTGGACACGA

CTCTCCTGAGTTAGCCTCTTCC

SQSTM1/p62

GCTCTTCGGAAGTCAGCAAACC

GCAGTTTCCCGACTCCATCTGT

PINK1

CGACAACATCCTTGTGGAGTGG

CATTGCCACCACGCTCTACACT

OPTN

AGGTGGAGAGACTTGAAGTCGC

TCCTCGCTGTCTGCTTCTCAGT

P21

TCGCTGTCTTGCACTCTGGTGT

CCAATCTGCGCTTGGAGTGATAG

GAPDH

CATCACTGCCACCCAGAAGACTG

ATGCCAGTGAGCTTCCCGTTCAG

  1. ATG12 autophagy-related gene 12, BECN1 Beclin 1, BNIP3 BCL2 interacting protein 3, CDK5 cyclin-dependent kinase 5, GAPDH glyceraldehyde-3-phosphate dehydrogenase, LC3 microtubule-associated protein light chain 3, OPTN optineurin, PINK1 PTEN-induced kinase 1, SQSTM1/p62 sequestosome 1.
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