Fig. 1
From: Restorative potential of ciliary body cells in a retinal ganglion cell degeneration model
Generation and characterization of neurospheres from mouse CB cells. (A) BrdU immunofluorescence (red) in 5 days neurospheres generated from cells isolated from the ciliary body of 2 months-old mice. Nuclei were counterstained with DAPI (blue). (B) Quantification of proliferation rate in growing neurospheres (growth Rate = diameter(t1)/diameter (t1-1 day) – 1) X 100). ***p < 0.001. (C) Upper panels show neurosphere derived cells from the ciliary body of GFP mice one and nine days after the induction of differentiation. Bottom panels show GFP fluorescence from the same image. Note that many cells in the neurosphere are pigmented and/or positive for GFP but they lose the pigment and/or GFP as they leave the neurosphere to differentiate. (D) Immunofluorescence for βIII-tubulin/Tuj1 and calretinin (red) in cells derived from CB-neurospheres 7 days after induction of differentiation. Nuclei were counterstained with DAPI (blue).
