Fig. 4
From: Comparison of antibody-scTRAIL Fc fusion proteins with varying valency for EGFR and TRAIL receptors
Cell death induction assay of hexavalent and trivalent TRAIL molecules on Colo205 and HCT116 cells. (A) Hexavalent TRAIL molecules on Colo205. (B) Trivalent TRAIL molecules on Colo205. (C) Hexavalent TRAIL molecules on HCT116. (D) Trivalent TRAIL molecules on HCT116. scTRAIL-antibody fusion proteins were titrated at a 1:3 dilution between concentrations starting at 1 nM (2 + 2 formats) or 10 nM (0 + 2 formats), 50,000 cells/well, detection by crystal violet staining. n = 3, mean ± SD.
