Table 1 Software and webservers used in the study and their respective weblinks.

Sequence retrieval

NCBI - GenBank

https://www.ncbi.nlm.nih.gov/genbank/

Sequence alignment

Clustal omega

https://www.ebi.ac.uk/jdispatcher/msa/clustalo

B cell epitope prediction

IEDB - BepiPred linear epitope 2.0

http://tools.iedb.org/bcell/

CD4 + epitope prediction

IEDB - MHC II binding prediction

http://tools.iedb.org/mhcii/

CD8 + epitope prediction

IEDB - Proteasomal cleavage/ TAP transport/ MHC class I combined predictor tool

http://tools.iedb.org/processing/

Antigenicity prediction

VaxiJen v.2.0

https://www.ddg-pharmfac.net/vaxijen/VaxiJen/VaxiJen.html

Allergenicity prediction

AllergenFP v.1.0

https://ddg-pharmfac.net/AllergenFP/

Conservancy prediction

IEDB conservancy analysis

http://tools.iedb.org/conservancy/

Cross-reactivity

BLASTp

https://blast.ncbi.nlm.nih.gov/

Physicochemical analysis

ExPasy ProtParam

https://web.expasy.org/protparam/

Secondary structure prediction

PSIPRED V3.3

http://bioinf.cs.ucl.ac.uk/psipred/

GOR4

https://npsa-pbil.ibcp.fr/NPSA/npsa_gor4.html

Tertiary structure modelling

AlphaFold3

https://alphafoldserver.com/

Tertiary structure refinement

Galaxy Refine server

https://galaxy.seoklab.org/cgi-bin/submit.cgi?type=REFINE

Structural validation

ERRAT

https://saves.mbi.ucla.edu/

Structure visualization

PyMOL

https://www.pymol.org/

Molecular docking

ClusPro server

https://cluspro.org/home.php

Receptor cleaning

ChimeraX

https://www.rbvi.ucsf.edu/chimerax/

Molecular dynamics

Desmond simulation package from Schrödinger LLC

https://www.schrodinger.com/platform/products/desmond/

PRODIGY

https://rascar.science.uu.nl/prodigy/

Normal mode analysis

iMODS

https://imods.iqf.csic.es/

Immune response profile

C-ImmSim server

https://kraken.iac.rm.cnr.it/C-IMMSIM/index.php

Codon optimization

Vector builder

https://en.vectorbuilder.com/tool/codon-optimization.html

Insilico cloning

Novobuilder tool

https://www.novoprolabs.com/plus/novobuilder/