Fig. 8

Visualization of alternative splicing (AS) events in the transcriptome of the anterior pituitary cells (APc) under the influence of visfatin. (A) Volcano plot showing the differences in AS events frequency between control and visfatin-treated groups. The X-axis represents ΔPSI-values for each AS event, and the Y-axis shows the negative decimal logarithm of the adjusted p-value. The horizontal line indicates the negative logarithmic adjusted p-value cut-off (q = 1.3), while the vertical lines represent the ΔPSI-value cut-off (ΔPSI>|0.1|). Different colours of dots represent different types of AS events: blue — alternative 3′ splicing sites (A3SS), yellow — alternative 5′ splicing sites (A5SS), red — mutually exclusive exons (MXE), dark green — retention intron (RI), and purple — skipping exon (SE), grey – not significant (Not Sig).