Fig. 2

ANO1 is expressed in urethral smooth muscle cells. (A) Representative images of urethral SMC (USMC) captured in brightfield and at different wavelengths (488 nm and 555 nm) to show double immuno-labelling for α-actin (red) and ANO1 (green) from WT male urethra (n = 3) and (B) female urethra (n = 3). (C) Representative images of corpus cavernosum SMC captured in brightfield and at different wavelengths (488 nm and 555 nm) to show double immuno-labelling for α-actin (red) and ANO1 (green) (n = 3) (D) Representative images of bladder SMC captured in brightfield and at different wavelengths (488 nm and 555 nm) to show immuno-labelling for α-actin (red) and a lack of ANO1 labelling in the same cell (green) (n = 3). Scale bar: 20 μm in all panels.