Fig. 3 | Scientific Reports

Fig. 3

From: ANO1 channels are expressed in mouse urethral smooth muscle but do not contribute to agonist or neurally evoked contractions

Fig. 3

The specific and potent ANO1 inhibitor Ani9 does not affect urethral contractions evoked by phenylephrine (PE) or basal tone. (A) Representative isometric tension trace showing male USM contractions induced by increasing phenylephrine (PE) concentrations (30 nM − 30 µM), (# represents washing). (B) In the same tissues shown in panel A, after 30 min incubation with Ani9 (3 µM), the cumulative PE doses were reapplied. (# represents washing). (C) Representative contractile trace of Ani9 (3 µM) on basal male urethral tone. (D) Summary effects of Ani9 on PE dose responses curves in male USMC (n = 6). (E) Summary effects of Ani9 on basal resting tone in males USMC (n = 6).

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