Fig. 4
From: Establishment of enterotype-specific antibodies for various diagnostic systems
Western blotting of target bacteria with the monoclonal antibodies (mAbs) established in this study to detect protein binding patterns. To identify whether distinct proteins were detected by the mAbs we isolated, we used them in western blotting against (A) Phocaeicola vulgatus JCM5826, (B) Faecalibacterium duncaniae JCM31915, and (C) Segatella copri JCM13464, as representatives of human gut microbiome enterotypes. Representative images are shown for the bands of lysates of P. vulgatus, F. duncaniae, or S. copri strains treated with selected clones. Data are representative of two independent experiments. The entire gel and blot are provided in the supplementary materials.
