Fig. 2
From: RNA demethylase ALKBH5 regulates cell cycle progression in DNA damage response
Knockdown of ALKBH5 enhances the efficiency of DSB repair post X-ray exposure. (A) Schematic of the reporter system for the analysis of HR-mediated DSB repair. (B) Schematic of the reporter system for the analysis of NHEJ-mediated DSB repair. (C) Landscape of GFP + cells determined by FACS analysis. Cells transfected with HR reporter or NHEJ reporter together with mCherry-expressing internal control plasmid were treated with X-ray (10 Gy). The expression of GFP and Cherry were determined by flowcytometry after 48 h. The proportion of GFP + cells in Cherry + cells was obtained as a relative frequency. The framed area indicates the percentage of GFP+/Cherry+. (D) Quantification of the frequency of HR-mediated DSBR in control and ALKBH5-KD cells. The values are indicated as Mean ± SD. Data were compared statistically using the two-tailed Student’s t-test. (*, p < 0.05). (E) Quantification of the frequency of NHEJ-mediated DSBR in in control and ALKBH5-KD cells. The values are indicated as Mean ± SD. Data were compared statistically using the two-tailed Student’s t-test. (*, p < 0.05; ***, p < 0.001).
