Fig. 3
From: RNA demethylase ALKBH5 regulates cell cycle progression in DNA damage response
Knockdown of ALKBH5 increases m6A modification and expression levels of mRNAs. (A) Consensus motifs of m6A sites in control and ALKBH5-KD cells exposed to X-rays. (B) Metagene plot of mRNA m6A peak distribution in control and ALKBH5-KD cells following X-ray irradiation. (C) Violin plot displaying RNA m6A abundance in the control and ALKBH5 knockdown cells after X-ray exposure. POI, peak over input. The upper and lower quartiles, as well as the median, are indicated for each group. Statistical significance was determined using the Mann-Whitney test. (****, p < 0.0001). (D) Heatmap of RNA expression in whole cell, nuclei, and cytoplasm of control and ALKBH5 knockdown cells after X-ray exposure. (E) Violin plot displaying RNA expression changes of ALKBH5 target and non target after ALKBH5 knockdown following X-ray exposure. The upper and lower quartiles, as well as the median, are indicated for each group. Statistical significance was determined using the Mann-Whitney test. (ns, not significant; ****, p < 0.0001).
