Fig. 2
From: Automatic quantification of disgust reactions in mice using machine learning
Experimental setup for taste reactivity test in mice. (A) Solution was infused intraorally through an implanted tube. Orofacial and somatic behaviors during infusions were video-recorded in a bottom-up view. (B) This scheme shows the Experimental timeline. Experiment 1,2 (Innate): On Day 1, mice received intraoral infusions of Quinine solution as habituation. On Day 2 (test day), the mice received the same infusions as those performed on Day 1, and the behaviors of the mice during the infusions were video-recorded. In Experiment 1, infusions were performed twice for a total of 1 min each, with a 1 min interval in between. In Experiment 2, the infusions were continuous for 2 min, without intervals. Experiment 3 (Innate): On Day 1, mice received intraoral infusions of quinine solution, and shortly thereafter received an i.p. injection of physiological saline as a control treatment for the learned groups described below. The same procedure was repeated on Day 4. On Day 6 (test day), the mice received intraoral infusions of quinine solution, and their behaviors during the infusions were video-recorded. In Experiment 3, infusions were performed twice for a total of 1 min each, with a 1 min interval in between. Experiment 4,5 (Learned): On Day 1, mice received intraoral infusions of saccharin solution, and shortly after that, they received an i.p. injection of LiCl solution (1st conditioning). The same procedure was repeated on Day 4 (2nd conditioning). On Day 6 (test day), the mice received intraoral infusions of saccharin solution, and their behaviors during the infusions were video-recorded. In Experiment 4, infusions were performed twice for a total of 1 min each, with a 1 min interval in between. In Experiment 5, the infusions were continuous for 2 min without intervals.
