Fig. 2

qPCR test performed in a Seraph-treated septic patient. The bloodstream pathogen load was assessed before (orange curve) and at the end (blu curve) of the hemoperfusion session (4 h). The Cq values, defined as the fractional number of cycles that were needed for the RFU to reach the quantification threshold, were inversely related to the pathogen burden. The difference of the two Cq values, defined as ∆Cq, assessed at the beginning and at the end of the hemoperfusion session, is a surrogate marker of the bloodstream pathogen burden reduction, reflecting the capacity of the adsorber filter to remove the pathogen. Abbreviations: Cq: quantification cycle; LOD: limit of detection; RFU: fluorescence; ∆Cq: differences between the two Cq values revealed before and after the Seraph treatment.