Fig. 1

CLEVER-1 is expressed on malignant myeloid cells. (A) STAB1 RNA expression in 39 AML cell lines, presented in a heatmap as log2 values, derived from DepMap and cell surface CLEVER-1 protein expression in 11 AML cell lines, detected with two antibodies that recognize different epitopes of CLEVER-1 and presented as GeoMean in a heatmap. (B) STAB1 RNA expression in primary samples representing different hematological (pre)malignancies and cell types, derived from the Hemap database. AML = acute myeloid leukemia, CML = chronic myeloid leukemia, MM = multiple myeloma, MP = myeloproliferative disease, LP = lymphoproliferative disease, T-ALL = T-cell acute lymphoblastic leukemia, TCL = T cell lymphoma, pre-B-ALL = pre-B-cell acute lymphoblastic leukemia, BCL = B cell lymphoma, CLL = chronic lymphocytic leukemia. (C) Total expression of surface or intracellular CLEVER-1 protein (in CD45 + leukocytes) and in different myeloid cell populations (bubble plot) of primary AML and MDS samples. Each column represents one sample. CLEVER-1 expression (Median Fluorescence Intensity, MFI or Geometric Mean Intensity, GeoMean) is indicated by the color of the rectangle (in CD45 + leukocytes) or circle (in different myeloid cell populations). Circle size reflects the size of the population as % from CD45 + cells in each sample. Lack of cell population specific CLEVER-1 expression is due to the cell population being too small for analysis (< 5% of CD45 + in control treated sample at 48 h). ‘Blasts’ (CD45loSSClo) and ‘monocyte-like’ (CD45hiSSClo/mid) cell populations are defined based on CD45-SSC, ‘CD34+’ (CD45 + CD34+) is a subpopulation of blasts and monocyte subpopulations are based on CD14/CD16 expression (Fig. S1). (D) Summary data for CLEVER-1 expression in different cell populations of FIMM cohort samples. Each dot is one sample, line shows cell population mean and grey-colored populations are sub-populations of blasts or monocyte-like cells. P-values are based on Kruskal-Wallis and Dunn’s test.