Fig. 2
From: Monitoring the rate and variability of somatic genomic alterations using long-read sequencing
Density plots of SNV allele frequencies before filtration (top) and after aggressive filtration (bottom) to enrich for somatic variants. The large majority of all SNVs (99.95%) are removed by the filtering step. The unfiltered SNVs show a typical distribution of frequencies associated with germline variants (broad peak around 50% frequency associated with heterozygous variants and a narrow peak around 100% comprising homozygous variants and sequencing errors), whereas the filtered SNVs are enriched for low-intermediate frequencies (10–40%).
