Figs. 12

(a) Photomicrograph of the control group’s testicular slice demonstrating the closely packed, primarily spherical seminiferous tubules (ST) and the Leydig cell-containing interstitial tissue (arrow) between them. The lumen of the seminiferous tubule contains the sperms (S) (H&E X 200, scale bar = 100uµm). (b): Photomicrograph of testicular section of AcL group (A), AsL group (B), FPO group (C), and SHPO group (D) showing the same histological picture as the control group (H&E X 200, scale bar = 100uµm). (c): Photomicrograph of the RHPO group’s testicular section demonstrating the disordered and apparent reduction in spermatogenic cells. These cells feature pyknotic, tiny, darkly pigmented nuclei (yellow arrow) and vacuolated cytoplasm. The tubules have open areas and vacuolated portions (V). No sperm is visible in the tubules’ wide lumen (Lu) despite their size. The interstitial cells of Leydig (L) seem to be less in number, and the basement membrane of the seminiferous tubules seems to be broken and discontinuous (black arrow) (H&E X 200, scale bar = 100uµm). (d): Photomicrograph of testicular section of AcL + RHPO group (A), AsL + RHPO group (B), and AcL + AsL + RHPO group (C) demonstrates that the testis’s histological structure is almost identical to that of the control group, but vacuolated areas (V) can be detected within the tubules in AsL + RHPO group (B). The interstitial cells of the Leydig (L) are increased in number in AsL + RHPO group (B) and AcL + AsL + RHPO group (C) (H&E X 200, scale bar = 100uµm).