Fig. 3
A, B Western blot and quantification of phosphorylated (p) and total (t) levels of HER2, EGFR, HER3, ERK, and Akt in NCIN87 cells. β-actin was used as a loading control. The following conditions were analyzed: control, statin, neratinib, statin/neratinib, T-DM1, T-DM1/lovastatin, T-DM1/neratinib, and T-DM1/lovastatin/neratinib. NCIN87 cells were incubated for 48 h with 20 nM T-DM1 alone or after lovastatin (4 h) and/or neratinib (3 h) treatments17. Graph bars (B) show quantification of Western blots. Full-length blots are included in the Supplementary Information document. Statistical analyses were performed comparing the treatment groups T-DM1/statin, T-DM1/neratinib, and T-DM1/statin/neratinib to T-DM1 only. Bars, n = 3 per group, mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001 based on a Student’s t test.
