Table 3 Sites of sponge collection and TB history in European bison (Bison bonasus).

From: Sponge-based environmental DNA detection as a useful tool in monitoring Mycobacterium tuberculosis complex markers in European bison (Bison bonasus)

Site

Type of herd

TB history

Antibodies against P22 (MTC) in European bison21

MTC DNA (IS6110 and IS1081 targets) detection in sponge samples (percent positive samples (95% CI) (number of positive/sample size)

Bałtów

Captive

In spring 2024 in two animals positive result in PCR swab one animal positive in IGRA (unpublished data).

0% (0/2)

0% (0/9)

Białowieża Forest

Free-living

TB never reported.

6.9% (95% CI: 2.78–5.13)

7.14% (95% CI: 3.7–31.71) (1/14)

Białowieża Forest

Captive

TB never reported.

7.9% (95% CI: 2.19–20.79)

0 (0/2)

Bieszczady Mountains

Free-living

Two TB outbreaks in European bison: 1997–2001 and 2010–2013. TB confirmed also in other species. Last TB case in wild boar (not published) in 2024.

5.9% (95% CI: 1.63–16.36)

29.4% (95% CI: 12.38–54.41) (5/17)

Bieszczady, Muczne

Captive

TB has never been reported in a captive herd, but it has been reported in wildlife species in the surrounding area.

12.5% (95% CI: 2.27–37.16)

50% (95% CI: 24.27–75.73) (6/12)

Gołuchów

Captive

TB never reported.

0%

0% (0/3)

Knyszyńska Forest

Free-living

TB has been never been reported. However, positive results in IGRA test have been detected in last five years (unpublish data)

10.8% (95% CI: 3.78–25.38)

21.4% (95% CI: 6.12- 50.00) (3/14)

Pszczyna

Captive

TB never reported.

5.3% (95% CI: 0.95–17.99)

14.3% (95% CI: 0.74–55.42) (1/7)

Smardzewice

Captive

TB in herd between 2013–2022. In December 2022 the last animal from herd was culled. Sampling was conducted due to plans for introducing European bison again to Smardzewice.

Not included.

16.7% (95% CI: 0.86–58.86) (1/6)