Fig. 6
From: A novel luciferase-based assay for quantifying coronavirus-induced syncytia
Quantitative evaluation of FCoV spike protein-induced syncytia in Fcwf-4 cells stably expressing ssTau-split Gluc. (a) FCoV S-induced syncytia in Fcwf-stable Tau cells. Fcwf-stable Tau cells were transfected with empty (Vect), tag-free (WT), PA-tagged, or GFP-tagged S expression vectors. Syncytia are surrounded by a dashed line in light microscopy images obtained 24 hpt. Scale bar: 100 μm. (b) Gluc activity of the cells shown in panel a, as determined 24 hpt. The data are expressed as the mean ± SD, with raw values of each well indicated as circles. The luciferase values relative to those of WT are indicated at the top of each bar.
